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An0102_rev032202.qxd (page 2)

VYDAC® Columns for
USP and Pharmaceutical Analyses

The VYDAC SELECTAPORE™ is recommended for USP analyses. The Penicillin V
contributes stability and long column life.
analysis. Variations in selectivity based on The specially treated base silica minimizes tailing for alkaline analytes, and the 300 Å pore size provides uniform surface access for large multi-ring molecules. Performance analyses of pharmaceuticals, on the other hand, analytical conditions and suitability Column: VYDAC 218WP54 (C18, 300 Å, 5 µm,4.6 mm ID x 250 mm). Detection: 254 nm. Flow: 1.0 mL/min. Mobile phase: 60:40water:ACN with 1% HOAc. Isocratic. Trisulfapyrimidines
Norethindrone and ethinyl estradiol
Ampicillin
Column: VYDAC 218WP54 (C18, 300 Å, 5 µm, Column: VYDAC 218WP54 (C18, 300 Å, 5 µm, Column: VYDAC 218WP54 (C18, 300 Å, 5 µm, 4.6 mm ID x 250 mm). Detection: 254 nm. 4.6 mm ID x 250 mm). Detection: 200 nm. 4.6 mm ID x 250 mm). Detection: 254 nm. Flow: 1.0 mL/min. Mobile phase: 86:13:1 Flow: 1.0 mL/min. Mobile phase: 20 mM Flow: 1.0 mL/min. Mobile phase: 909:80:10:1 water:ACN:glacial acetic acid. Isocratic. KH2PO4, pH 6.0, 35% ACN. Isocratic. water:ACN:1M KH2PO4 :1N acetic acid. Isocratic. b-Estradiol
Prednisolone acetate
Fludrocortisone
Column: VYDAC 218WP54 (C18, 300 Å, 5 µm, Column: VYDAC 218WP54 (C18, 300 Å, 5 µm, Column: VYDAC 218WP54 (C18, 300 Å, 5 µm, 4.6 mm ID x 250 mm). Detection: 205 nm. 4.6 mm ID x 250 mm). Detection: 254 nm. 4.6 mm ID x 250 mm). Detection: 254 nm. Flow: 1.0 mL/min. Mobile phase: 51:49 Flow: 1.0 mL/min. Mobile phase: 40% ACN Flow: 1.0 mL/min. Mobile phase: 45:55 Grace Vydac is a business unit of W. R. Grace & Co.-Conn.
Application Note #0102
Separation of Barbiturates on Small-Pore
and Wide-Pore Reversed-Phase Columns

convulsants by exerting depressant effects reversed for phenobarbital/butalbital and for secobarbital/mephobarbital due to dif- ferences in the mobile phase. Differences The five barbiturates were also separated differences in selectivity were observed.
Ordering Information
Cat. No. Description
Column. SELECTAPORE 90M. MonomericC18. 90 Å. 5 µm. 4.6 mm ID x 250 mm.
Figure 2. Separation of five barbiturates on VYDAC reversed-phase columns. Columns: (a & b)
VYDAC 201SP54 (monomeric C18, 90 Å, 5 µm, 4.6 mm ID x 250 mm). (c) VYDAC 238TP54

Column. Polymeric C18. 300 Å. 5 µm. 4.6 mm ID x 250 mm.
(monomeric C18, 300 Å, 5 µm, 4.6 mm ID x 250 mm). (d) VYDAC 218TP54 (polymeric C18, 300 Å,5 µm, 4.6 mm ID x 250 mm). Mobile phases: (a) A = water. B = ACN. Gradient from 30% to 60% B over 20 minutes. (b,c,&d) A = 50 mM KH2PO4, pH 2.51, 20% ACN. B = 90% ACN. Gradient from Column. SELECTAPORE 300P. PolymericC18. 300 Å. 5 µm. 4.6 mm ID x 250 mm.
To place an order, call (800) 247-0924, fax (888) 244-6610 or contact your local Grace Vydac distributor.
Grace Vydac
17434 Mojave Street Hesperia, CA 92345 www.gracevydac.com
Copyright 2002 Grace Vydac. All rights reserved. Prices and specifications subject to change without notice. VYDAC® is a registered trademark and SELECTAPORE is a trademark of The Separations Group.

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