BACKGROUND RECOMMENDED SECONDARY REAGENTS
MAP (mitogen-activated protein) kinases play a significant role in many bio-
To ensure optimal results, the following support (secondary) reagents are
logical processes, including cell adhesion and spreading, cell differentiation
recommended: 1) Western Blotting: use goat anti-rabbit IgG-HRP: sc-2004
and apoptosis. p38α, p38β and p38γ, also known as MAPK14, MAPK11 and
(dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible goat anti-
MAPK14, respectively, each contain one protein kinase domain and belong
rabbit IgG-HRP: sc-2030 (dilution range: 1:2000-1:5000), Cruz Marker™
to the MAP kinase family. Expressed in different areas throughout the body
Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent:
with common expression patterns in heart, p38 proteins use magnesium as
sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunopreci-
a cofactor to catalyze the ATP-dependent phosphorylation of target proteins.
pitation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/ 2.0 ml).
Via their catalytic activity, p38α, p38β and p38γ are involved in a variety of
3) Immunofluorescence: use goat anti-rabbit IgG-FITC: sc-2012 (dilution range:
events throughout the cell, including signal transduction pathways, cytokine
1:100-1:400) or goat anti-rabbit IgG-TR: sc-2780 (dilution range: 1:100-1:400)
production and cell proliferation and differentiation. The p38 proteins are
with UltraCruz™ Mounting Medium: sc-24941. 4) Immunohistochemistry: use
subject to phosphoryation on Thr and Tyr residues, an event which is thought
ImmunoCruz™: sc-2051 or ABC: sc-2018 rabbit IgG Staining Systems.
to activate the phosphorylated protein. REFERENCES
1. Han, J., et al. 1993. Endotoxin induces rapid protein tyrosine phosphoryla-
tion in 70Z/3 cells expressing CD14. J. Biol. Chem. 268: 25009-25014.
2. Brewster, J.L., et al. 1993. An osmosensing signal transduction pathway
3. Nishida, E., et al. 1993. The MAP kinase cascade is essential for diverse
signal transduction pathways. Trends Biochem. Sci. 18: 128-131.
p38α/β (H-147): sc-7149. Western blot analysis of
p38α/β (H-147): sc-7149. Immunoperoxidase staining
p38α expression in Jurkat (A) and KNRK (B) whole cell
of formalin-fixed, paraffin-embedded pancreas tumor
showing cytoplasmic and nuclear staining.
p38α/β (H-147) is a rabbit polyclonal antibody raised against amino acids213-360 mapping at the C-terminus of p38α of human origin. SELECT PRODUCT CITATIONS
1. Chen, G., et al. 2000. The p38 pathway provides negative feedback for
Ras proliferative signaling. J. Biol. Chem. 275: 38973-38980.
Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azideand 0.1% gelatin.
2. Schultheiss, U., et al. 2001. TRAF6 is a critical mediator of signal trans-
duction by the viral oncogene latent membrane protein 1. EMBO J. 20:
Available as agarose (sc-7149 AC) conjugate for immunoprecipitation, 500 µg/
3. Kumar, N. and Dey, C.S. 2002. Gliclazide increases insulin receptor tyro-
APPLICATIONS
sine phosphorylation but not p38 phosphorylation in insulin-resistantskeletal muscle cells. J. Exp. Biol. 205: 3739-3746.
p38α/β (H-147) is recommended for detection of p38α and p38β of mouse,rat and human origin by Western Blotting (starting dilution 1:200, dilution
4. Kumar, N. and Dey, C.S. 2002. Metformin enhances insulin signalling in
range 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total
insulin-dependent and -independent pathways in insulin resistant muscle
protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilu-
cells. Br. J. Pharmacol. 137: 329-336.
tion range 1:50-1:500), immunohistochemistry (including paraffin-embedded
5. Khurana, A. and Dey, C.S. 2003. p38 MAPK interacts with Actin and
sections) (starting dilution 1:50, dilution range 1:50-1:500) and solid phase
modulates filament assembly during skeletal muscle differentiation.
ELISA (starting dilution 1:30, dilution range 1:30-1:3000).
6. Hsu, M.F., et al. 2004. Mechanisms of the influence of magnolol on
Positive Controls: Jurkat whole cell lysate: sc-2204.
eicosanoid metabolism in neutrophils. Biochem. Pharmacol. 67: 831-840 .
7. Chen, C.J., et al. 2004. Inhibition of inducible nitric oxide synthase
expression by baicalein in endotoxin/cytokine-stimulated microglia.
Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of
shipment. Non-hazardous. No MSDS required.
8. Stoecklin, G., et al. 2004. MK2-induced tristetraprolin:14-3-3 complexes
prevent stress granule association and ARE-mRNA decay. EMBO J. 23:
RESEARCH USE
For research use only, not for use in diagnostic procedures. Santa Cruz Biotechnology, Inc.
1.800.457.3801 831.457.3800 fax 831.457.3801 Europe
+00800 4573 8000 49 6221 4503 0 www.scbt.com
REYATAZ ® Pharmacokinetics The pharmacokinetics of atazanavir were evaluated in healthy adult volunteers and in HIV-infected patients after administration of REYATAZ (atazanavir sulfate) Capsules (atazanavir sulfate) 400 mg once daily and after administration of REYATAZ 300 mg with ritonavir 100 mg once daily (see Table 3). Table 3: Steady-State Pharmacokinetics of Atazanavir in Healt
Für die Agenda: Das Schulhaus Weinberg wird im Zusammenhang mit der Renova-tion des Schulhauses Milchbuck ab Schuljahr 2007/ 2008 ander-weitig genutzt. Deshalb zügeln folgende Lehrpersonen aus dem Schulhaus Weinberg ins Schulhaus Scherr: • Silvia Schneider mit ihrer jetzigen 2. Klasse und der Fachlehrerin • Clarissa Baroni mit einer neuen, zusätzlichen 1. Klasse. • Fredi Schni
BACKGROUND