Antibiotic Susceptibility Testing: Genital Tract Isolates
The Ontario Association of Medical Laboratories’ (OAML) Guidelines on Antibiotic Susceptibility Testing have been developed to provide ordering physicians with a clear and concise reference respecting the testing and reporting of microbiological susceptibilities for patients in the community. It should be recognized that resistance patterns will change and that the guidelines will be subject to periodic revision. The guidelines represent the consensus thinking of a panel of experts in the field of microbiology and, while they are generally applicable, they are no substitute for sound clinical judgement. Microbiological susceptibility testing is performed with reference to National Committee for Clinical Laboratory Standards (NCCLS) standards* and in keeping with the recommendations of the Laboratory Proficiency Testing Program. Recommendations:
Major Pathogens
Susceptibility Test
Test and Report
N. gonorrhoeae:?-lactamase test ?Neisseria gonorrhoeae (only in pre-
N. gonorrhoeae:?-lactamase test Cervicitis
N. gonorrhoeae:?-lactamase test Bartholinitis
N. gonorrhoeae:?-lactamase test Genital Ulcers
for testing of H. ducreyi. Refer to * Performance Standards for Antimicrobial Susceptibility Testing, NCCLS Document M100-S9, Performance Standards for Antimicrobial Disk Susceptibility Tests, NCCLS Document M2-A6 and Methods for Dilution Antimicrobial Susceptibility for Bacteria that Grow Aerobically, NCCLS Document M7-A4.
The Ontario Association of Medical Laboratories
The Ontario Association of Medical Laboratories (OAML) represents the community-based laboratory sector in
Its mission is to promote excellence in the provision of laboratory services and, as an essential component of the health
care system, to contribute to shaping the future of health care in Ontario.
The OAML encourages the highest level of professional and ethical integrity and technical excellence among laboratory
owners, operators and staff in the provision of laboratory services for the benefit of the people of Ontario.
Guidelines for Clinical Laboratory Practice
The OAML, through its Quality Assurance and physicians. There may be additional educational Clinical Laboratory Practice Committee, co-ordinates materials produced, if it is thought that they might be the development and dissemination, implementation useful, and these are distributed with the guideline. and evaluation of Guidelines for Clinical Laboratory The comments of end users are essential to the
development of guidelines which will encourage A proposed Guideline is developed by a working
adherence. You are strongly encouraged to submit group of the Committee with the participation of your comments on this or on any other OAML outside experts. The proposed guideline is then submitted to the Committee as a whole and to a Professional Advisory Group who provide an overall review of the document. The comments of the Quality Assurance and Clinical Laboratory Practice Committee and the Professional Advisory Group are incorporated into a revision of the guideline and this Ontario Association of Medical Laboratories draft is submitted to laboratory Medical Directors, professional associations and other representatives of end users for additional comment. The document is revised in light of these comments and submitted to the OAML Board of Directors for approval. Approved guidelines are distributed to Community-
based Laboratories and by them to their client Members of the Expert Panel
Mount Sinai Hospital and The Toronto Medical Head, Division of Microbiology and Immunology Sunnybrook and Women’s College Health Sciences Centre, The OAML gratefully acknowledges the contributions of the members of the Expert Panel and others who have contributed their expertise, advice and technical support to the development and review of this guideline. This guideline has been reviewed by and comments have been received from representatives of the Laboratory Medicine, Geriatrics and General and Family Practice Sections of the Ontario Medical Association and of the Laboratory Proficiency Testing Program of the



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