New drugstore levitra australia online viagradirect.net with a lot of generic and brand medicament with cheap price and fast delivery.
Macedonian Journal of Medical Sciences. 2012 Oct 15; 5(3):324-327.
Actinomyces Odontolyticus - Associated Bacteremia
Anika Považan1, Anka Vukelic1, Nevena Secen2, 3, Danica Sazdanic-Velikic2, Daliborka Bursac2
1Institute for Pulmonary Diseases of Vojvodina, Center for Microbiology, Immunology and Virology, Sremska Kamenica, Serbia; 2Institute forPulmonary Diseases of Vojvodina, Pulmonary Oncology Clinic, Sremska Kamenica, Serbia; 3Faculty of Medicine, University of Novi Sad,Department of Internal Medicine, Novi Sad, Serbia
Považan A, Vukelic A, Secen N,
are part of the oral microflora of humans. Actinomyces odontolyticus
Sazdanic-Velikic D, Bursac D. Actinomyces
associated infections are exceptionally rare, presenting an endogenous infection originating from
- Associated Bacteremia. Maced JMed Sci. 2012 Oct 15; 5(3):324-327. http://
mucous membranes. Immunodeficiency is often complicated by severe opportunistic bacterial infections
leading to critical condition of the patient. We report a case of an immunosuppressed patient with fever
Key words: Actinomyces odontolyticus;
and Actinomyces odontolyticus
bacteremia. The patient poorly responded to the applied antimicrobial
Dr. Anika Považan. Institute
for Pulmonary Diseases of Vojvodina, Center for
Microbiology, Immunology and Virology, Put
doktora Goldmana 4, Sremska Kamenica 21204,
Serbia. Phone: +381/21/4805348. Fax: +381/21/
527960. E-Mail: firstname.lastname@example.org
Received: 03-Apr-2012; Revised: 08-May-2012;Accepted: 09-May-2012; Online first:23-Sep-2012
2012 Považan A. This is an open-
access article distributed under the terms of the
Creative Commons Attribution License, which
permits unrestricted use, distribution, and
reproduction in any medium, provided the original
author and source are credited.
The author have declared
that no competing interests exist.
[1, 3]. Most frequently, Actinomyces israelii
enters thebody either through a damaged skin or mucosa, or via
is a Gram-positive,
inhalation . Actinomyces odontolyticus
facultative anaerobic bacterium [1, 2]. It was initially
exceptionally rare, presenting an endogenous infection
isolated from dental caries in 1958. As other Actinomyces
arising most frequently from mucous membranes.
species, this organism colonizes the oral cavity . The
Clinically the disease caused by Actinomyces
most significant pathogen of the genus Actinomyces
closely resembles to the disease caused
which causes actinomycosis of the
by Actinomyces israelii
and other representatives of the
cervico-facial, thoraco-pulmonary and abdominal region
genus. Pulmonary infections , bacteremia  and
Považan et al. Actinomyces Odontolyticus - Associated Bacteremia
abscess of various organs [1, 5-7] caused by thispathogen have been reported.
A 62-year male patient was admitted to hospital
to enlighten the etiology of the lesion presented on hischest X-ray, localized in the right upper lobe. He reporteda two-month history of back pains, fatigue, exhaustion,appetite and body mass losses (8 kg in two months).
Having noticed a tumorous lesion on the patient’s chestX-ray (Figure 1, 2), chest computed tomography (CT)was performed delineating an infiltration of 27 mm in
histopathological finding of the biopsy samples takenfrom the right upper lobe confirmed adenocarcinoma.
Due to the right jaw and right lower leg pains, scintigraphyof the skeleton was performed, detecting pathologicalhyperfixation foci in the projection of the right parietalbone, along all spinal column segments, bilaterally in theribs, shoulder region bones, left humerus diaphysis,sacroiliac joint and proximal femur tips. Due to theadvanced stage of the disease, the chemotherapy
Figure 1: PA chest X-ray at the first day of hospitalization.
regimen with paclitaxel/carboplatin was recommended,accompanied with bisphosphonate administration.
diameter localized in the right upper lobe, with adjacent
However, on the 10th hospital day, the patient’s condition
reactive pneumonitis and a few micronodular subpleural
deteriorated, developing fever (390C) and blood
lesions contralaterally, the 9th rib fracture with infiltrated
leukocytosis (35.5 x 109/L), with predominating
pleura and subpleural fat tissue, as well as enlarged
granulocytes (96.5); procalcitonin levels also increased
retrocaval, bronchopulmonary lymph nodes on the right,
(2.58 ng/ml), as well as nitrogenous substances in the
and those in the subcarinal region (Figure 3).
blood (blood urea 28.2 mmol/l, creatinine 311 ìmol/L).
Gas analysis of the arterial blood at rest revealed asevere partial respiratory insufficiency (SaO2 81.7%,PaO2 6.16 kPa, PaCO2 5.41kPa, pH 7.396). The chestX-ray finding was presented with a bronchopneumonicinfiltration paracardially on the right (Figure 4).
Parenteral antibiotic treatment was initiated,
amoxicillin with clavulanic acid. After two days, due toelevated procalcitonin levels (138.24 ng/ml) andworsened medical condition, the therapy was switchedto ceftazidime and ciprofloxacin, according to the NCCN(National Comprehensive Cancer Network) guidelinesfor immunosuppressed patients and hospital-acquiredpneumonia. In the mean time, one set of blood cultures
Figure 2: Profile chest X-ray at the first day of hospitalization.
Maced J Med Sci. 2012 Oct 15; 5(3):324-327.
result. The identification was performed by anaerobicbacterial identification systems (BBL Crystal IdentificationSystems Gram Anaerobe Kit, Beckton Dickinskon, USA)and Actinomyces odontolyticus
Antimicrobial susceptibility testing could not be donedue to technical difficulties. Similar cases ofimmunocompromised patients with Actinomycesodontolyticus
infection suggested the efficiency oftetracyclines besides penicillin and cephalosporins inthe treatment of such infection . It was decided to
Figure 4: PA chest X-ray with pneumonic shadow.
include doxycycline in the treatment.
Procalcitonin level and white blood count tended
was sent for microbiological examination. Blood cultures
to decrease, and the control chest X-ray finding showed
where cultivated in the automated BacT/Alert system
an initial regression of the pneumonic infiltration in the
using both anaerobe and aerobe liquid media. The
instrument indicated positive cultures after the 24 hour-incubation. Gram staining of the liquid cultures revealed
However, on the 10th day of antimicrobial therapy,
short Gram-positive bacilli. The blood cultures were
the medical state of the patient worsened, developing
subcultured on the blood agar and MacConkey agar in
the symptoms of endogenous intoxication, resulting in a
aerobic conditions and on Shaedler agar in anaerobic
lethal outcome on the same day. The patient’s dental
conditions. After 48h of incubation on the blood and
status was normal, did not reveal any possible infection
Shaedler agar, a noticeable growth was registered.
Colonies were small, smooth and slightly whitish. Gramstain of the culture revealed Gram-positive short rodsresembling diphteroids in pattern. The oxidase and
catalase tests were performed, producing a negative
is a Gram-positive
facultative anaerobic, non sporulating, non-motilebacterium [1, 2]. The Gram-smear may be presentedwith shorter bacilli resembling diphteroids in pattern.
Small, whitish, smooth or slightly granular colonies appearon the blood agar, developing dark red pigment in 2-14days [1, 4]. Pigmentation is best recognized when thecultures, following the primary anaerobic isolation, areleft on the room temperature. Actinomyces odontolyticus
grows well on the CDC and Brucella agar .
catalase and oxidase tests, reduces nitrates to nitrites,and does not grow at pH 5.5 . The final identificationcan be done by identification systems based onbiochemical reactions , or by polymerase chain reaction(PCR), analyzing the 16S rRNA gene sequence .
Moreover, the 16S rRNA gene sequence is recomendedin final identification of Actinomyces
species , but it isstill not routinely used in clinical microbiology laboratories.Actinomyces odontolyticus
is susceptible to penicillin,cephalosporins, tetracycline, clindamycin,chloramphenicol and erythromycin .
The identification of Actinomyces odontolyticus
in blood cultures of the patient with advanced lung
Figure 5: Control PA chest X-ray after antibiotics.
Považan et al. Actinomyces Odontolyticus - Associated Bacteremia
malignancy was made according to microscopic, cultural
and biochemical characteristics of the subculture grown
1. Chao CT, Liao CH, Lai CC, Hsueh PR. Liver abscess due to
on the blood and Shaedler agar and using the BBL
Actinomyces odontolyticus in an immunocompetent patient.
The representatives of the genus Actinomyces
2. Cone LA, Leung MM, Hirschberg J. Actinomyces
are commensals of the oral cavity microflora which
bacteremia. Emerg Infect Dis. 2003;12:1629–
participate in development of dental caries, periodontitis
and other infections . Actinomyces odontoyticus
3. Mabeza GF, Macfarlane J. Pulmonary actinomycosis. Eur
infections are endogenous, originating from mucous
membranes . Actinomyces odontoyticus
growspredominantly on the surface of the tongue in supra and
4. Takiguchi Y, Terano T, Hirai A. Lung abscess caused by
subgingival regions , which may account for a normal
. Intern Med.
5. Simpson AJ, Das SS, Mitchelmore IJ. Polymicrobial brainabscess involving Haemophilus paraphrophilus and
Infections caused by Actinomyces odontolyticus
Actinomyces odontolyticus. Postgrad Med J. 1996;72:297–8.
are rarely found, more frequently affectingimmunosuppressed patients, predominantly middle-aged
6. Sugano S, Matuda T, Suzuki T, Makino H, Iinuma M, Ishii K,
males . The reported patient is a middle-aged male
Ohe K, Mogami K. Hepatic actinomycosis: case report and
review of the literature in Japan. J Gastroenterol.1997;32:672–6.
7. Woo PC, Fung AM, Lau SK, Hon E, Yuen KY. Diagnosis of
pelvic actinomycosis by 16S ribosomal RNA gene sequencingand its clinical significance. Diagn Microbiol Infect Dis.
complicated with severe infections caused byopportunistic bacteria, such as the reported infection
8. Cavallaro JJ, Wiggs LS, Miller JM. Evaluation of the BBLCrystal Anaerobe Identification System. J Clin Microbiol.
induced by Actinomyces odontolyticus
in the patient with
advanced lung malignancy. Such infections often havea variable outcome despite the applied antimicrobial
9. Drobni M, Hallberg K, Öhman U, Birve A, Persson K,
treatment. Better understanding of the pathogenetic
Johansson I, et al. Sequence analyses of fimbriae subunit FimA
mechanism of such opportunistic agents may help in
proteins on Actinomyces naeslundii
genospecies 1 and 2 and
developing some other treating strategies.
with variant carbohydrate bindingspecificities. BMC Microbiol. 2006; 6: 43.
Maced J Med Sci. 2012 Oct 15; 5(3):324-327.
NZ Microbiological Society Conference 2013 "OUR CHANGING PLANET - THREATS AND OPPORTUNITIES" Draft programme - still subject to change Monday, 25 Nov Waikato University, Lecture Block 'S', Room SG.01 Registration Welcome Mixer CHAIRMAN: Dr Jonathan Broadbent (University of Otago) Preventing bacterial destruction of teeth through community water fluoridation
U N T E R S U C H U N G S B E F U N D Auftraggeber: Prüfsubstanz: Natives Olivenöl extra ex Griechenland Chargen-Bez.: Probeneingang: PRÜFERGEBNISSE: Prüfparameter Ergebnis Grenzwert Prüfparameter Ergebnis Grenzwert Beurteilung: Gemäß den vorliegenden Untersuchungsergebnissen handelt es sich bei der Probe um "natives Olivenöl e